DETECTION OF PIG DNA FRAGMENTS IN HALAL UNLABLED LIPSTICK SAMPLES USING CONVENTIONAL PCR

From a Muslim perspective, it is very important to know the content, raw materials, and processing of materials used in cosmetic products used. One type cosmetics that most often lipstick. However, many lipsticks circulating Indonesia are not equipped with halal logo. ingredients for lipstick pork derivatives. These pig derivatives can be detected using PCR. Based on this background, study aims test presence DNA samples have been certified market 4 combinations fragments coding primers by conventional PCR method. Five commercial were selected purposive sampling. isolation was carried out according Wizard Promega Universal Kit. The process temperature optimization as follows: Predenaturation 98oC: 2 minutes, denaturation 95oC: 30 seconds, Annealing 61oC: Extension 72oC: 40 Postextension 3 minutes. results showed 5 tested kinds primer combination, none suspected contain DNA. difficult step sample detection process. Even though result negative, necessary carry further tests which become Gold Standard DNA-based testing Real Time